Microbiology Theory Paper

Q1 Which of the following are nutritional bacterial growth requirements?
A. Carbon
B. Nitrogen
C. Ph
D. Temperature
E. Osmotic effects

Q2 Production of indole from tryptophan is a characteristic of:
A. Enterobacter cloacae
B. Escherichia coli
C. Shigella dysentriae
D. Shigella sonnei

Q3 The standard wire loop used for urine culture has a capacity of:
A. 0.1ml
B. 0.01ml
C. 0.001ml
D. 0.0001ml

Q4 Neonatalophthalmia is caused by :
A. Neisseria meningitidis
B. Neisseria gonorrhoea
C. Streptococcus pyogenes
D. Staphylococcus aureus

Q5 The following sample must be collected in a sterile container.
A. Sputum
B. Urine
C. Stool
D. Blood

Q6 Cocci arranged in groups of eight are known as:
A. Diplococci
B. Tetrads
C. Sarcina
D. Aerococcus
E. Gaffkya

Q7 Neutral red indicator is used in:
A. Methyl red
B. Urea broth
C. MacConkey agar
D. Triple Sugar Iron agar

Q8 Members of the Enterobacteriaceae are:
A. Gram-positive rods
B. Non-lactose fermenters
C. Catalase positive
D. Oxidase positive
E. Ferment glucose with production of gas

Q9 Germ tube test is used in the diagnosis of :
A. Staphylococcus aureus
B. Candida albicans test
C. Streptococcus pyogenes
D. Streptococcus agalactiae


Q10 Durham tube is useful in demonstration of:
A. Bile solubility
B. Carbohydrate fermentation with gas production
C. Motility of microorganisms
D. Urea hydrolyzation
E. Oxidation

Q11 Staphylococcus aureus food poisoning is as a result of:
A. Leucocidin
B. Catalase
C. Enterotoxin
D. Exfoliatin
E. Hyaluronidase

Q12 V. cholerae transmission is mainly through:
A. Inhalation
B. Ingestion of infected food and water
C. Contact
D. Blood transfusion

Q13 Which of the following media is both selective and differential?
A. Blood agar
B. McConkey media
C. nutrient broth
D. nutrient agar
E. selenite F

Q14 The following is the most commonly used method in antimicrobial susceptibility testing:
A. Macfarland 0.5 standard
B. Epsilon test
C. Micro broth dilution
D. Stokes method
E. Kirby Bauer method

Q15 The following is an important property of viruses:
A. They divide by binary fission
B. They contain a cell wall and a cytoplasmic membrane
C. They either have DNA or RNA as genetic material but never both
D. They contain flagella for locomotion

Q16 The following media is suitable for stocking microorganisms for reference
A. Tryptone soy broth
B. Nutrient agar
C. Selenite F
D. Blood agar

Q17 Cultures and specimens from microbiology lab should be disposed of by
A. Incineration
B. Placing them in a highly infectious waste bag or container
C. Placing them in a sharps container
D. Placing them in a non-infectious waste bag or container

Q18 The following is transport media for a stool specimen
A. Amies
B. Stuart
C. Cary Blair
D. Alkaline peptone water
E. Selenite F

Q19 Darkfield microscopy is commonly applied in the diagnosis of
A. Syphilis
B. Salmonellosis
C. Typhoid fever
D. brucellosis

Q20 Diseases which may be associated with Staphylococcus species include
A. Endocarditis
B. Undulant fever
C. Gastroenteritis
D. Bloody diarrhea