Microbiology Theory Paper

Q1 Which of the following is a substitute for crystal violet used in gram-staining procedure?
(a) Methylene blue
(b) Bromocresol green
(c) Safranin
(d) Phenolphthalein

Q2 The cocci which mostly occur in single or pairs are:
(a) Streptococci
(b) Diplococci
(c) Tetracocci
(d) None of these

Q3 Which of the following medium are used to differentiate the colonies of Vibrio cholerae and V. parahaemolyticus?
(a) Alkaline bile salt
(b) Thiosulphate-citrate-bile-sucrose
(c) MacConkey
(d) All of the above

Q4 Which of the following can be identified by milk ring test?
(a) Brucellosis
(b) Salmonellosis
(c) Bovine tuberculosis
(d) All of these

Q5 Which of the following specimen is not expected to have any bacteria in normal conditions?
(a) Urethral swab
(b) Sputum
(c) Pus
(d) Cerebrospinal fluid

Q6 Which of the folowing methods is not suitable for sterilization of bacteriological media?
(a) Tyndillization
(b) Autoclaving
(c) Steaming at 100oC
(d) disinfecting in Lysol

Q7 The most pathogenic Brucella spp for man is:
(a) B melitensis
(b) B abortus
(c) B suis
(d) B canis

Q8 Which of the following disinfectant is NOT effective against viruses?
(a) Hydrogen peroxide
(b) Hypochlorite
(c) Formaldehyde
(d) methylated spirit

Q9 Salmonella typhi is associated with?
(a) Botulinum
(b) Brucellosis
(c) Amoebic dysentery
(d) Enteric fever

Q10 Cryptococcus neoformans is an opportunistic pathogen commonly isolated from?
(a) Slool
(b) Urine
(c) CSF
(d) Blood

Q11 Which of the following is best used for long term storage of microbial specimens when carried out properly?
(a) Storage in a freezer at -10°C
(b) Storage in a freezer at ultra low temperatures (-70°C)
(c) Storage in a refrigerator on an agar slant
(d) Storage on a petri plate at room temperature

Q12 The concept of putting microbes to help clean up the environment is called:
(a) Pasteurization
(b) Bioremediation
(c) Fermentation
(d) Biolistics

Q13 The nature of the poliovirus given for oral vaccination (satin vaccine) as part of the eradication programme is:
(a) Heat killed virus
(b) Live attenuated strains of all three immunological types
(c) Small dosage of wild-type live viruses
(d) Formalin-inactivated viruses

Q14 For most bacteria, the optimum pH for growth lies between:
(a) 6.5-7.5
(b) 3.5-4.5
(c) 4.5-5.5
(d) 5.5-6.5

Q15 Suspension cultures consist of cells and cell aggregates, growing dispersed in:
(a) Liquid medium
(b) Solid nutrient medium
(c) Solid or liquid medium
(d) None of these

Q16 Which of the following is not the characteristic of a growth curve?
(a) Shows development of microbial population under relatively stable environmental conditions
(b) Plotted with logarithmic numbers
(c) Graphs numbers of microbes versus time
(d) Each growth curve consists of four distinct phases

Q17 The organism which obtains their energy from chemicals are designated as:
(a) Prototrophs
(b) Chemotrophs
(c) Organotrophs
(d) Autotrophs

Q18 Which of the following media is selective for Salmonella?
(a) Thiosulphate citrate bile salt agar
(b) Wilson and Blair’s brilliant-green bismuth sulphite agar medium
(c) Bile salt agar
(d) All of the above

Q19 Xylose lysine deoxycholate (XLD) agar is better selective medium than the deoxycholate citrate agar (DCA) because:
(a) It is selective
(b) It imparts better growth
(c) It contains a high salt concentration
(d) It is less inhibitory to S. Dysentriae and S. flexneri

Q20 The viruses that attack bacteria are:
(a) Bacterial viruses
(b) Bacterial pathogens
(c) Bacteriophages
(d) Various