Histology and Cytology Theory Paper

Q.1.     Which of the following are accessory organs are involved in the digestion of food?

a) Liver

b) Heart

c) Kidney

d) Pancreas

e) Mouth


Q.2.     Functions of the epithelium include:

a) Excretion

b) Growth

c) Secretion

d) Absorption

e) Digestion


Q.3.     The prophase stage of Mitotic cell division is characterised by:

a) The appearance of 2 daughter cells

b) Constriction of the cytoplasm

c) Condensation of chromosomal material

d) Division of centrosome

e) The disappearance of the spindle


Q.4.     10% formal saline consists of:

a) Sodium chloride

b) Formal chloral

c) Formaldehyde

d) Calcium oxide

e) Hydrochloric acid


Q.5.     Histo-chemical fixatives are used to preserve:

a) Lipids

b) Enzymes

c) Hormones

d) N.A.

e) C.L.


Q.6.     Picric Acid is a peculiar chemical because:

a) It can be used as a clearing agent

b) It can be used as a fixative in Bollin’s fluid

c) It can be used as a dehydrant

d) It can be used as a counterstain in Van Gieson’s solution

e) It can be used as a buffer


Q.7.     The following can be used to prevent the formation of acid formaldehyde pigments:

a) Potassium permanganate

b) Peracetic acid

c) Oxalic acid

d) Buffered formalin solution at pH – 7.0

e) Methyl benzoate


Q.8.     Softening of hard tissues is done by use of:

a) Ammonium oxalate

b) Glycerol

c) 70% Alcohol

d) 4% Phenol

e) Sulphuric acid


Q.9.     Double embedding of tissues involves the use of:

a) Gelatin

b) Celloidin

c) Paraffin Wax

d) Synthetic resin

e) Low Viscosity Nitrocellulose


Q.10.   The embedding medium for demonstration of fat is:

a) Epoxy resin

b) Ester wax

c) Water-soluble wax

d) Gelatin

e) Beeswax


Q.11.   The following are factors involved in tissue impregnation or infiltration except:

a) Agitation

b) Heat

c) Viscosity

d) Vaccum

e) pH


Q.12.   The following is/are dehydrating agents except:

a) Dioxane

b) Acetone

c) Alcohol

d) Xylene

e) Propanol


Q.13.   Which of the following is/are characteristics of a good decalcifying agent?

a) Complete removal of calcium ions

b) Damage tissue fibres

c) Act as a fixative

d) Allows subsequent treatment of tissue

e) Impairment of subsequent treatment of tissue


Q.14.   The disadvantages of Peveny’s fluid as a decalcifier are:

a) It causes no hardening of tissues

b) It is a gentle decalcifier

c) Decalcification time must be controlled carefully to avoid any damage to


d) It is rapid and therefore suitable for urgent work

e) A chemical test can not be used to assess the endpoint of decalcification

because of PP+ is formed when ammonia is added to Perany’s fluid even in absence of calcium


Q.15.   The rocking microtome is mainly designed for cutting:

a) Celloidin – embedded tissue blocks

b) Paraffin wax embedded tissue blocks

c) Synthetic resin embedded tissue blocks

d) Gelatin embedded tissue blocks

e) Agar embedded tissue blocks


Q.16.   Manual knife sharpening homes include:

a) Arkansas

b) Belgium yellow

c) Aluminium oxide

d) Strop

e) Knife back



Q.17.   Common tissue section adhesives include:

a) DPX

b) Colophonium resin

c) Damar balsam

d) Starch paste

e) Mayer’s egg albumen


Q.18.   Which of the following is used as an adhesive for microtome knife sharpening?

a) Mercuric chloride

b) Aluminium oxide

c) Lead oxide

d) Sodium borohydride

e) Picric acid


Q.19.   Sections may crumble on cutting.  This may be due to:

a) Dirty embedding medium

b) Wax being too soft

c) The angle of tilt being too great

d) Ante-medium not properly removed

e) Damaged knife-edge


Q.20.   Argentaffin granules also referred to as Kulschitzy cells are normally found in:

a) Adrenal medulla

b) Lieberkuhn

c) Mucous coat of appendix

d) Spleen

e) The small intestine in the crypts of Lieberkuln


Q.21.   Asbestos rods seen microscopically in tissues are:

a) Endogenous pigments

b) Exogenous   pigment

c) ZN positive

d) Perl’s Prussian positive

e) Insoluble in alcohol


Q.22.   Mucopolysaccharides are demonstrated by:

a) A.S

b) Weigerts

c) Silver preparations

d) Verhoeff’s

e) Celestin blue



Q.23.   Chloral hydrate is used as in Mayer’s haematoxylin:

a) Stain

b) Mordant

c) Preservative

d) Oxidizer

e) Solvent


Q.24.   Elastic fibres after staining with Verhoeff’s Iron haematoxylin; stain:

a) Green

b) Deep red

c) Colourless

d) Black

e) Yellow


Q.25.   Paraffin wax, cut tissue sections are floated in:

a) Soapy water

b) 10 – 20% alcohol

c) 20% Ether

d) Water bath at 10 – 15°C below M.P+ of wax used

e) Water bath at 10 – 15°C above melting P+ of wax used


Q.26.   The following types of preservatives can be used during the preparation of aqueous

Mounting media:


Gum Arabic

Phenol crystals

Cane sugar

Sodium nitrate


Q.27.   Water-soluble wares are equivalent to:

a) Gelatin

b) Celloidin

c) Polyethene glycols

d) Dioxane

e) V.N.


Q.28.   Drum sticks are bar bodies protruding from the nucleus of:

a) Blood cells

b) Semen cells

c) Buccal cavity

d) Vaginal cavity

e) Tear calls


Q.29.   75% of the sperm cells are contained in the first ………………. Of each ejaculation emission:

a) ¼

b) ¾

c) 1/3

d) 2/7

e) 1/8


Q.30.   The power of a Lens to reveal details is referred to as:

a) Virtual image

b) Real image

c) Resolving power

d) Principal focus

e) Numerical aperture


Q.31.   Nicol Prisms are employed in:

a) Dark ground microscopy

b) Phase-contrast microscopy

c) Electron microscopy

d) Polarising microscopy

e) Interference microscopy


Q.32.   Ayre spatula is used:

a) For preparing sputum

b) For preparing all types of Histological smears

c) For preparing an eye for Histological research

d) Taking cervical smears

e) For removing materials for gynaecological smears for Papanicolaou